Situ Pcr

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Real-time PCR/Temp - Real-time PCR is a specific type of Quantitative PCR (Q-PCR) which give results in real-time. Q-PCR is a modification of PCR used to rapidly measure the quantity of PCR product, thus is an indirect method for quantitatively measuring starting amounts of DNA, cDNA or RNA.

Real-time PCR - Real-Time PCR, also called quantitative (real-time) PCR, is a method of simultaneous DNA quantification and amplification. It is the real-time version of Q-PCR (Quantitative PCR).

Touchdown PCR - Touchdown PCR or Touchdown style PCR is a method of PCR (polymerase chain reaction) by which degenerate primers will avoid amplifying nonspecific sequence. The temperature at which primers anneal during a cycle of PCR determines the specificity of annealing.

RT-PCR - RT-PCR (Reverse Transcription-PCR) is a modification of PCR in which an RNA strand is first reverse transcribed into its DNA complement or cDNA, followed by amplification of the resulting DNA using PCR. This can either be a 1 or 2 step process.


Suggested Web Sites

In Situ PCR on Plant Material - Protocol for in situ PCR and RT-PCR, from the web site of the Botanical Institute of University of Copenhagen, Denmark.

Colony PCR protocol - Detailed protocol from the web site of the Department of Biology, University of Michigan, USA.

Enterococcus Colony PCR - Step by step colony PCR protocol from the Enterococcus Research Site, University of Oklahoma web, USA.

Source: BazSites.net

Web Links

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