Restriction Sites

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Restriction sites - Restriction sites, or restriction recognition sites, are particular sequences of nucleotides that are recognized by restriction enzymes as sites to cut the DNA molecule. The sites are generally palindromic, and a particular enzyme may cut between two nucleotides within its recognition site, or ...

Endonuclease - Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain. Restriction endonucleases (Restriction Enzymes) cleave DNA at specific sites, and are divided into three categories, Type I, Type II, and Type III, according to their mechanism of action.

Sub-cloning - Sub-cloning is the method by which two distinct gene(s) or gene fragments are fused together by means of restriction sites. Typically, a genetic insert from one vector is inserted into another vector.

Multiple cloning site - A multiple cloning site (MCS) is a short segment of DNA which contains many (usually 10+) restriction sites - a standard feature of engineered plasmids. Extremely useful in biotechnology, bioengineering, and molecular genetics, MCSs let a biotechnologist insert a piece of DNA or several pieces of DNA into the region of the MCS.


Suggested Web Sites

Religion Explorer - Provides a copy of the Open Directory categories on religion, with added site previews by Thumshots. Also uses Aspseek technology to provide a search engine restricted to sites listed in those categories.

The Production Code of the Motion Picture Industry (1930-1968) - For four decades, American theatrical movies were governed by a self-policing agency which restricted the content that audiences would see. This web site catalogs instance of content that was shaped by "The Code" or which circumvented or violated the restrictions.

Pharma Life - Commercial site providing online resources for pharmacists, mainly for trade purposes. Some of the site is restricted to registered users only.

Source: BazSites.net

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